Comprehensive programming options, a large touchscreen, and intuitive analysis software make it easy to obtain. No service engineer required no recalibration necessary instrument automatically detects and identifies block. Realtime ready custom qpcr assays can be selected for any human, mouse, or rat targets of your choice. May 17, 20 for todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected. Roche today announced the global launch of new software updates, including advanced high resolution melting analysis and qualitative gene detection modules for the light cycler 96 and new support features for the lightcycler nano system. A lightcycler probe system consists of a pair of singlestranded fluorescentlabeled oligonucleotides. Contact your roche representative for any additional questions. Roche lightcycler 96 biotecon diagnostics biotecon. Lightcycler 96 realtime pcr system from roche life science duration. Software will calculate the standard curve slope, efficiency, error, r2, and yintercept relquant if not already done open experimental file on lightcycler 96 user software. The instrument the lightcycler instrument figure from roche diagnostics is an extremely fast thermocycler with online fluorescence detection. Roche launches new software updates for its lightcycler 96 and lightcycler nano pcr systems. Operation training and onsite assistance are provided. New innovative lightcycler 96 application and instrument software creates a simple analysis workflow with easy import and export functions, email notifications.
Lightcycler 96 instrument for todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. Roche lightcycler ii laboratory thermal pcr system. Roche lightcycler 480ii384 pcr w thermocycler 96s 2. Work economically, adapting workflows to your format and throughput needs. New innovative lightcycler 96 application and instrument software creates a. Roche lightcycler 480 garvan institute of medical research. Lightcycler 480 multiwell plates clear, 96 and 384 well. Lightcycler 480 realtime pcr system from roche applied. The lightcycler system 3 instruments 4 lightcycler 2. For todays academic researcher labs, the new roche lightcycler 96 system.
System component 1 lightcycler instrument sample carousel for o 1. Use the upper right quick search via catalog number or product name. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. Roche lightcycler 480 operators manual department of cell. Tomastarcak aplikacnapodpora cobas 6000, cobas 8000, cobas integra 400 plus, cobas e 411 roche customer support center. Supported by a broad range of software tools, realtime pcr based analysis can be performed in 32 capillaries, interchangeable 96 384well plates or using the novel, unique 1536well format. Axygen lightcycler 480 compatible pcr plates, 96well 96well plate is designed to fit the roche lightcycler 480 brand. Service manual light cycler please note that this is only a. Use of the roche lightcycler instrument in a realtime pcr for trichomonas vaginalis in urine samples from females and males. With a total of 6 detection channels, this compact cycler offers the highest flexibility and allows single color and multiplex testing. Axygen lightcycler 480 compatible pcr plates, 96well 96. Roche lightcycler 480 ii real time pcr system for 96 well plates.
Oligo probe 1 is labeled at the 3 end with a donor reporter and oligo probe 2 is labeled at its 5 end with one of a few available acceptor reporters. A 96 384 microtiter format lightcycler appeared and a 1536well version will. Conforms to the european directive for in vitro diagnostic medical devices 9879ec in combination with the lightcycler 2. Intuitive touchscreen and powerful analysis modules. Service manual light cycler please note that this is only. Data were analyzed with lightcycler software by the fit point method to minimize noise and obtain the best correlation coefficient possible between standards. The lightcycler instrument of roche molecular biochemicals, mannheim, ge rmany, is a thermocycler for the rapid analysis of pcr applications. Compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis, mirna research, and relative quantification of target genes. Supported by a broad range of software tools, realtime pcr based analysis can be performed in 32 capillaries, interchangeable 96384well plates or using the novel, unique 1536well format. Roche introduces thermal shift assay application for lightcycler 1536 instrument new capability provides highthroughput protein melting solution for us biotech, pharmaceutical markets. Discover this compact and smart new member of the roche lightcycler realtime pcr system. However, roche diagnostics reserves the right to make any changes necessary without notice as part of ongoing product development. Jan 31, 20 lightcycler 96 realtime pcr system from roche life science duration. All assays are functiontested and enable fast and efficient expression profiling of your genes of interest.
The versatile, platebased lightcycler 96 system helps you achieve fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and snp analysis compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis. Eurofins dna quantification and snp detection on gold standard platforms. This lot comes with everything shown in the photos, to include. Lightcycler technology in molecular diagnostics ncbi nih. Accurate results expected from a lightcycler system even with a gradient. It enables the user to monitor the amplification of the pcr product simultaneously in realtime allowing the accurate quantification of target sequences. The main switch is located at the back of the instrument. Trichomonas vaginalis is the agent of a highly prevalent sexually transmitted infection sti that can result in vaginitis, urethritis, and preterm birth. The lightcycler 480 from roche is available for quantitative realtime pcr analysis and it can perform methylation analysis and high resolution melting analysis. The software is compatible with the regulations for electronic records subpart b. Roche molecular biochemicals lightcycler operators manual. Customizable modular system concept building on the principles of the wellestablished, performanceproven lightcycler software 4. Warranty information on warranty conditions are specified in the sales contract. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples.
Lightcycler roche life science realtime ready overview. Roche lightcycler 96 pcr system software updates labonline. The widely known 32capillary roche lightcycler was released in 1998. Roche launches new software updates for its lightcycler 96. Evaluation of two commercial realtime pcr assays for.
Roche lightcycler 480ii384 pcr w thermocycler 96 s 2. It offers fast and accurate analysis that impresses with its intuitive and userfriendly software. Roche diagnostics 04729692001 lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils. Roche diagnostics 04729692001 lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils catalog no. Turn on the computer linked to the instrument and log into window xp. The result of more than a decade of roche experience developing highperformance qpcr systems is now accessible for any research lab. Review and cite roche lightcycler protocol, troubleshooting and other methodology information contact experts in roche lightcycler to get answers. Realtime pcr lightcycler instruments roche molecular diagnostics has introduced the lightcycler instruments, the fastest thermocyclers with online monitoring of pcr based on fluorescence detection.
Use of the roche lightcycler instrument in a realtime pcr. Roche applied science lightcycler 480 realtime pcr system. The lightcycler 96 system delivers data that makes you a. These are low profile microplates compatible with many thermal cyclers, but especially suitable for the roche lightcycler 480. The 96 well block for the f plate format or the 384well block for g plate format. Roche introduces thermal shift assay application for. Innovative design of optics and thermal block provide an ideal combination of accuracy, temperature homogeneity, and reproducibility for unbiased result research requires. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user. Modify, adapt, translate, reverse engineer, decompile or disassemble the software. This lightcyler 480 instrument quick guide was created by the roche diagnostics corporation commercial education department. The 96well block for the f plate format or the 384well block for g plate format.
Equipment in the immune monitoring lab is open to all investigators. The lightcycler 96 system is a midthroughput qpcr solution that enable research breakthrough. Roche has launched highresolution melting analysis and qualitative detection modules for the lightcycler 96 pcr system. For todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. Ideal for all levels of experience, the lightcycler 96 instrument provides an optimal combination of temperature homogeneity and data reproducibility. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is. Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96. Quantitycomponent1 lightcycler 480 instrument, 96wellsorlightcycler 480. Contact your roche representative for further information. I prepared the twostep cycling experiment with the following conditions. I have a problem with the analysis in lightcycler 480 software.
Additional features of the lightcycler 480 pcr system. To download magna pure 96 system new 2 ml and 4 ml protocols, please first register by completing this form. Roche lightcycler 480 ii inquire about availability includes computer loaded with lightcycler 480 software release 1. Operation by a roche service engineer is not necessary. The lightcycler 96 is a realtime pcr cycler for medium sample throughput. Speed time to results with advanced yet easytouse software. Lightcycler technology is the most innovative and rapid possibility for carrying out and simultaneously evaluating pcr experiments. Lightcycler 480 system 96 384 switch interchangeable thermal block cycler doityourself, fast loading help for convenience no service engineer required no recalibration necessary instrument automatically detects and identifies block. The lightcycler 96 module for highresolution melting analysis will be suitable for human genetic disease research applications. New software releases and user guides for the lightcycler 96 instrument. Used roche lightcycler 480 ii real time pcr system lab. Traditional methods of diagnosis, including wet preparation, can be unreliable. Vwr 96well pcr plates for roche lightcycler 480 vwr.
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